Human and Animal Exposure (HUMANE)

Human and Animal Exposure (HUMANE)

The HUMan and ANimal Exposure (HUMANE) Facility Core provides researchers with advanced facilities and expertise to study how environmental exposures affect health. By recreating real-world conditions under controlled settings, HUMANE makes it possible to investigate pollutants, climate stressors, and their combined impacts with scientific precision.

Core Directors

Core Focus

The core offers NIH CEED Center research members and non-members the services to perform research at the intersection of pollution and health. 

 

Controlled Exposure Facility (CEF)

    • A dedicated facility for both human and animal exposure studies in safe, precisely defined atmospheres.
    • Equipped with temperature and humidity control systems, the CEF can simulate extreme weather events such as heat waves, high-humidity conditions, or other climate-relevant scenarios.
    • These capabilities enable researchers to study how pollutants and climate stressors interact and affect health outcomes.
    • Systems to generate and characterize particle and gas phase pollutants from different sources.
    • Supports toxicology studies across a wide range of emerging and established environmental contaminants.

State-of-the-Art Monitoring:

    • Real-time and time-integrated instruments to measure gases and particles with high precision.
    • Ability to construct controlled atmospheres while limiting or evaluating potential confounders.

Study Design and Support:

    • Expertise in examining how exposures interact with variables like physical activity, psychosocial stress, and pre-existing health conditions.

Core Mission

The overall goal of HUMANE is to provide CEED investigators with access to human and animal exposure platforms and expertise in the design and execution of experiments utilizing these systems. For human studies, the Core works closely with the CEED Translational Research Support Core (TRSC) on participant protocols, recruitment, screening and selection, ensuring that studies have an adequate sample size for statistical power, appropriate analytical tools for the proposed experiment, and that safety protocols are approved by the IRB. TRSC members and EOHSI Environmental and Occupational Health Clinical Center (EOHCC) personnel provide clinical oversight for human participants before, during and after exposures. For animal studies, HUMANE works with individual CEED investigators on exposures, experimental design, and scientific rigor and IACUC approval. 

HUMANE Core Services

The room-sized exposure chamber is located in the EOHSI building. The CEF consists of a ~25 m3 stainless steel room, which is controlled for temperature, humidity, and air exchange rate. The temperature control ranges roughly from 55º to 95º ± 1ºF, and the relative humidity from approximately 15% to 85% ± 2%. This wide range of parameters will also be feasible after the renovation and will allow CEED researchers to use the CEF as a “Climate Simulator” recreating extreme weather conditions. 

The AEF is located in the EOHSI building  Both Plexiglas and stainless-steel chambers including BUXCO systems are available to CEED investigators for whole body and nose only rodent inhalation exposures. Temperature and humidity are monitored and recorded continuously throughout the exposures. Real time and time integrated systems for the physicochemical characterization of particles and other gaseous co-pollutants are used for a comprehensive exposure characterization.

Recent Core Publications

Cary CM, DeLoid GM, Yang Z, Bitounis D, Polunas M, Goedken MJ, Buckley B, Cheatham B, Stapleton PA, Demokritou P. Ingested Polystyrene Nanospheres Translocate to Placenta and Fetal Tissues in Pregnant Rats: Potential Health Implications. Nanomaterials (Basel). 2023 Feb 14;13(4):720. doi: 10.3390/nano13040720. PMID: 36839088; PMCID: PMC9965230.

Azimi P, Keshavarz Z, Lahaie Luna M, Cedeno Laurent JG, Vallarino J, Christiani DC, Allen JG. An Unrecognized Hazard in E-Cigarette Vapor: Preliminary Quantification of Methylglyoxal Formation from Propylene Glycol in E-Cigarettes. Int J Environ Res Public Health. 2021 Jan 6;18(2):385. doi: 10.3390/ijerph18020385. PMID: 33419122; PMCID: PMC7825490.

Singh D, Tassew DD, Nelson J, Chalbot MG, Kavouras IG, Tesfaigzi Y, Demokritou P. Physicochemical and toxicological properties of wood smoke particulate matter as a function of wood species and combustion condition. J Hazard Mater. 2023 Jan 5;441:129874. doi: 10.1016/j.jhazmat.2022.129874. Epub 2022 Sep 1. PMID: 36084462; PMCID: PMC9532370.

Contact

Jose Guillermo Cedeno-Laurent: memo.cedeno@rutgers.edu

Gediminas Mainelis: mainelis@envsci.rutgers.edu